2Y0S
Crystal structure of Sulfolobus shibatae RNA polymerase in P21 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-11-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 129.330, 195.909, 212.451 |
| Unit cell angles | 90.00, 105.73, 90.00 |
Refinement procedure
| Resolution | 29.858 - 3.800 |
| R-factor | 0.3024 |
| Rwork | 0.300 |
| R-free | 0.35390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2waq |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.514 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.990 |
| High resolution limit [Å] | 3.790 | 3.790 |
| Rmerge | 0.200 | 0.700 |
| Number of reflections | 100163 | |
| <I/σ(I)> | 9.1 | 2.1 |
| Completeness [%] | 99.4 | 96.9 |
| Redundancy | 7.2 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | pH 7.8 | ||
| 1 | 7.8 | pH 7.8 |
