Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2Y0S

Crystal structure of Sulfolobus shibatae RNA polymerase in P21 space group

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0000428	cellular_component	DNA-directed RNA polymerase complex
A	0003677	molecular_function	DNA binding
A	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
A	0005737	cellular_component	cytoplasm
A	0006351	biological_process	DNA-templated transcription
A	0008270	molecular_function	zinc ion binding
A	0016779	molecular_function	nucleotidyltransferase activity
A	0034062	molecular_function	5'-3' RNA polymerase activity
A	0046872	molecular_function	metal ion binding
B	0000428	cellular_component	DNA-directed RNA polymerase complex
B	0003677	molecular_function	DNA binding
B	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
B	0005737	cellular_component	cytoplasm
B	0006351	biological_process	DNA-templated transcription
B	0008270	molecular_function	zinc ion binding
B	0016779	molecular_function	nucleotidyltransferase activity
B	0032549	molecular_function	ribonucleoside binding
B	0034062	molecular_function	5'-3' RNA polymerase activity
B	0046872	molecular_function	metal ion binding
C	0000428	cellular_component	DNA-directed RNA polymerase complex
C	0003677	molecular_function	DNA binding
C	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
C	0005737	cellular_component	cytoplasm
C	0006351	biological_process	DNA-templated transcription
C	0016779	molecular_function	nucleotidyltransferase activity
C	0034062	molecular_function	5'-3' RNA polymerase activity
D	0000428	cellular_component	DNA-directed RNA polymerase complex
D	0003677	molecular_function	DNA binding
D	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
D	0005737	cellular_component	cytoplasm
D	0006351	biological_process	DNA-templated transcription
D	0016779	molecular_function	nucleotidyltransferase activity
D	0034062	molecular_function	5'-3' RNA polymerase activity
D	0046872	molecular_function	metal ion binding
D	0046983	molecular_function	protein dimerization activity
D	0051536	molecular_function	iron-sulfur cluster binding
D	0051538	molecular_function	3 iron, 4 sulfur cluster binding
E	0000428	cellular_component	DNA-directed RNA polymerase complex
E	0003676	molecular_function	nucleic acid binding
E	0003677	molecular_function	DNA binding
E	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
E	0005737	cellular_component	cytoplasm
E	0006351	biological_process	DNA-templated transcription
E	0006352	biological_process	DNA-templated transcription initiation
E	0016779	molecular_function	nucleotidyltransferase activity
F	0000166	molecular_function	nucleotide binding
F	0000428	cellular_component	DNA-directed RNA polymerase complex
F	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
F	0005737	cellular_component	cytoplasm
F	0006351	biological_process	DNA-templated transcription
F	0006352	biological_process	DNA-templated transcription initiation
F	0016779	molecular_function	nucleotidyltransferase activity
F	0030880	cellular_component	RNA polymerase complex
F	0044237	biological_process	cellular metabolic process
G	0000428	cellular_component	DNA-directed RNA polymerase complex
G	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
G	0005737	cellular_component	cytoplasm
G	0006351	biological_process	DNA-templated transcription
G	0016779	molecular_function	nucleotidyltransferase activity
H	0000428	cellular_component	DNA-directed RNA polymerase complex
H	0001054	molecular_function	RNA polymerase I activity
H	0001055	molecular_function	RNA polymerase II activity
H	0001056	molecular_function	RNA polymerase III activity
H	0003677	molecular_function	DNA binding
H	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
H	0005737	cellular_component	cytoplasm
H	0006351	biological_process	DNA-templated transcription
H	0006360	biological_process	transcription by RNA polymerase I
H	0006366	biological_process	transcription by RNA polymerase II
H	0006383	biological_process	transcription by RNA polymerase III
H	0016779	molecular_function	nucleotidyltransferase activity
H	0034062	molecular_function	5'-3' RNA polymerase activity
I	0000428	cellular_component	DNA-directed RNA polymerase complex
I	0003677	molecular_function	DNA binding
I	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
I	0005737	cellular_component	cytoplasm
I	0006351	biological_process	DNA-templated transcription
I	0016779	molecular_function	nucleotidyltransferase activity
I	0034062	molecular_function	5'-3' RNA polymerase activity
J	0000428	cellular_component	DNA-directed RNA polymerase complex
J	0003677	molecular_function	DNA binding
J	0005737	cellular_component	cytoplasm
J	0016779	molecular_function	nucleotidyltransferase activity
J	0070063	molecular_function	RNA polymerase binding
K	0000428	cellular_component	DNA-directed RNA polymerase complex
K	0003677	molecular_function	DNA binding
K	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
K	0005737	cellular_component	cytoplasm
K	0006351	biological_process	DNA-templated transcription
K	0016779	molecular_function	nucleotidyltransferase activity
K	0034062	molecular_function	5'-3' RNA polymerase activity
L	0000428	cellular_component	DNA-directed RNA polymerase complex
L	0003677	molecular_function	DNA binding
L	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
L	0005737	cellular_component	cytoplasm
L	0006351	biological_process	DNA-templated transcription
L	0016779	molecular_function	nucleotidyltransferase activity
L	0034062	molecular_function	5'-3' RNA polymerase activity
L	0046983	molecular_function	protein dimerization activity
M	0000428	cellular_component	DNA-directed RNA polymerase complex
M	0003677	molecular_function	DNA binding
M	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
M	0005737	cellular_component	cytoplasm
M	0006351	biological_process	DNA-templated transcription
M	0016779	molecular_function	nucleotidyltransferase activity
M	0034062	molecular_function	5'-3' RNA polymerase activity
M	0046983	molecular_function	protein dimerization activity
N	0000428	cellular_component	DNA-directed RNA polymerase complex
N	0003677	molecular_function	DNA binding
N	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
N	0005737	cellular_component	cytoplasm
N	0006351	biological_process	DNA-templated transcription
N	0008270	molecular_function	zinc ion binding
N	0016779	molecular_function	nucleotidyltransferase activity
N	0034062	molecular_function	5'-3' RNA polymerase activity
N	0046872	molecular_function	metal ion binding
O	0000428	cellular_component	DNA-directed RNA polymerase complex
O	0003677	molecular_function	DNA binding
O	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
O	0005737	cellular_component	cytoplasm
O	0006351	biological_process	DNA-templated transcription
O	0008270	molecular_function	zinc ion binding
O	0016779	molecular_function	nucleotidyltransferase activity
O	0034062	molecular_function	5'-3' RNA polymerase activity
O	0046872	molecular_function	metal ion binding
P	0000428	cellular_component	DNA-directed RNA polymerase complex
P	0003677	molecular_function	DNA binding
P	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
P	0005737	cellular_component	cytoplasm
P	0006351	biological_process	DNA-templated transcription
P	0008270	molecular_function	zinc ion binding
P	0016779	molecular_function	nucleotidyltransferase activity
P	0034062	molecular_function	5'-3' RNA polymerase activity
P	0046872	molecular_function	metal ion binding
Q	0000428	cellular_component	DNA-directed RNA polymerase complex
Q	0003677	molecular_function	DNA binding
Q	0005737	cellular_component	cytoplasm
Q	0016779	molecular_function	nucleotidyltransferase activity
Q	0070063	molecular_function	RNA polymerase binding
R	0000428	cellular_component	DNA-directed RNA polymerase complex
R	0003677	molecular_function	DNA binding
R	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
R	0005737	cellular_component	cytoplasm
R	0006351	biological_process	DNA-templated transcription
R	0008270	molecular_function	zinc ion binding
R	0016779	molecular_function	nucleotidyltransferase activity
R	0032549	molecular_function	ribonucleoside binding
R	0034062	molecular_function	5'-3' RNA polymerase activity
R	0046872	molecular_function	metal ion binding
S	0000428	cellular_component	DNA-directed RNA polymerase complex
S	0003677	molecular_function	DNA binding
S	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
S	0005737	cellular_component	cytoplasm
S	0006351	biological_process	DNA-templated transcription
S	0016779	molecular_function	nucleotidyltransferase activity
S	0034062	molecular_function	5'-3' RNA polymerase activity
S	0046872	molecular_function	metal ion binding
S	0046983	molecular_function	protein dimerization activity
S	0051536	molecular_function	iron-sulfur cluster binding
S	0051538	molecular_function	3 iron, 4 sulfur cluster binding
T	0000428	cellular_component	DNA-directed RNA polymerase complex
T	0003676	molecular_function	nucleic acid binding
T	0003677	molecular_function	DNA binding
T	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
T	0005737	cellular_component	cytoplasm
T	0006351	biological_process	DNA-templated transcription
T	0006352	biological_process	DNA-templated transcription initiation
T	0016779	molecular_function	nucleotidyltransferase activity
U	0000166	molecular_function	nucleotide binding
U	0000428	cellular_component	DNA-directed RNA polymerase complex
U	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
U	0005737	cellular_component	cytoplasm
U	0006351	biological_process	DNA-templated transcription
U	0006352	biological_process	DNA-templated transcription initiation
U	0016779	molecular_function	nucleotidyltransferase activity
U	0030880	cellular_component	RNA polymerase complex
U	0044237	biological_process	cellular metabolic process
V	0000428	cellular_component	DNA-directed RNA polymerase complex
V	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
V	0005737	cellular_component	cytoplasm
V	0006351	biological_process	DNA-templated transcription
V	0016779	molecular_function	nucleotidyltransferase activity
W	0000287	molecular_function	magnesium ion binding
W	0000428	cellular_component	DNA-directed RNA polymerase complex
W	0003677	molecular_function	DNA binding
W	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
W	0005737	cellular_component	cytoplasm
W	0006351	biological_process	DNA-templated transcription
W	0008270	molecular_function	zinc ion binding
W	0016779	molecular_function	nucleotidyltransferase activity
W	0034062	molecular_function	5'-3' RNA polymerase activity
W	0046872	molecular_function	metal ion binding
X	0000428	cellular_component	DNA-directed RNA polymerase complex
X	0003677	molecular_function	DNA binding
X	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
X	0005737	cellular_component	cytoplasm
X	0006351	biological_process	DNA-templated transcription
X	0008270	molecular_function	zinc ion binding
X	0016779	molecular_function	nucleotidyltransferase activity
X	0034062	molecular_function	5'-3' RNA polymerase activity
X	0046872	molecular_function	metal ion binding
Y	0000428	cellular_component	DNA-directed RNA polymerase complex
Y	0003677	molecular_function	DNA binding
Y	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
Y	0005737	cellular_component	cytoplasm
Y	0006351	biological_process	DNA-templated transcription
Y	0016779	molecular_function	nucleotidyltransferase activity
Y	0034062	molecular_function	5'-3' RNA polymerase activity
Z	0000428	cellular_component	DNA-directed RNA polymerase complex
Z	0001054	molecular_function	RNA polymerase I activity
Z	0001055	molecular_function	RNA polymerase II activity
Z	0001056	molecular_function	RNA polymerase III activity
Z	0003677	molecular_function	DNA binding
Z	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
Z	0005737	cellular_component	cytoplasm
Z	0006351	biological_process	DNA-templated transcription
Z	0006360	biological_process	transcription by RNA polymerase I
Z	0006366	biological_process	transcription by RNA polymerase II
Z	0006383	biological_process	transcription by RNA polymerase III
Z	0016779	molecular_function	nucleotidyltransferase activity
Z	0034062	molecular_function	5'-3' RNA polymerase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN A 1880

Chain	Residue
A	CYS58
A	THR60
A	CYS61
A	CYS68
A	HIS71

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 1881

Chain	Residue
A	CYS98
A	CYS101
A	VAL145
A	CYS146

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG A 1882

Chain	Residue
A	ASP456
A	ASP458
A	ASP460

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 2123

Chain	Residue
B	CYS1064
B	CYS1067
B	CYS1082
B	ILE1084

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ZN B 2124

Chain	Residue
B	HIS570
B	ASN572
B	GLU622

site_id	AC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE ZN B 2125

Chain	Residue
B	ASP418
B	THR420

site_id	AC7
Number of Residues	10
Details	BINDING SITE FOR RESIDUE F3S D 1001

Chain	Residue
D	CYS183
D	GLU185
D	VAL187
D	CYS203
D	THR204
D	LEU205
D	CYS206
D	GLU207
D	CYS209
D	ILE219

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN N 1065

Chain	Residue
N	CYS7
N	CYS10
N	CYS44
N	CYS45

site_id	AC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN O 1065

Chain	Residue
O	CYS7
O	CYS10
O	CYS44
O	CYS45

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN P 1049

Chain	Residue
P	CYS9
P	THR12
P	CYS26
P	CYS29

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN R 2123

Chain	Residue
R	CYS1064
R	CYS1067
R	CYS1082
R	ILE1084

site_id	BC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ZN R 2124

Chain	Residue
R	HIS570
R	ASN572
R	GLU622

site_id	BC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE ZN R 2125

Chain	Residue
R	ASP418
R	THR420

site_id	BC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE F3S S 1001

Chain	Residue
S	CYS183
S	GLU185
S	VAL187
S	CYS203
S	THR204
S	LEU205
S	CYS206
S	GLU207
S	CYS209
S	ILE219

site_id	BC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN W 1880

Chain	Residue
W	CYS58
W	THR60
W	CYS61
W	CYS68
W	HIS71

site_id	BC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN W 1881

Chain	Residue
W	CYS98
W	CYS101
W	VAL145
W	CYS146

site_id	BC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG W 1882

Chain	Residue
W	ASP456
W	ASP458
W	ASP460

site_id	BC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN X 1049

Chain	Residue
X	CYS9
X	THR12
X	CYS26
X	CYS29

Functional Information from PROSITE/UniProt

site_id	PS00446
Number of Residues	41
Details	RNA_POL_D_30KD RNA polymerases D / 30 to 40 Kd subunits signature. NAIRRaamlyvpvmSiddVyfieNnSplyDEiLAhRLALIP

Chain	Residue	Details
D	ASN26-PRO66

site_id	PS01110
Number of Residues	14
Details	RNA_POL_H_23KD RNA polymerases H / 23 Kd subunits signature. HYLVPKHevLsiDE

Chain	Residue	Details
H	HIS14-GLU27

site_id	PS01111
Number of Residues	15
Details	RNA_POL_K_14KD RNA polymerases K / 14 to 18 Kd subunits signature. TrYEiARvISaRAlQ

Chain	Residue	Details
I	THR28-GLN42

site_id	PS01112
Number of Residues	10
Details	RNA_POL_N_8KD RNA polymerases N / 8 Kd subunits signature. MIPIrCFTCG

Chain	Residue	Details
N	MET2-GLY11

site_id	PS01154
Number of Residues	32
Details	RNA_POL_L_13KD RNA polymerases L / 13 to 16 Kd subunits signature. IegEdHTLgNlIagtLrkisgVsfasYyqpHP

Chain	Residue	Details
L	ILE17-PRO48

site_id	PS01166
Number of Residues	13
Details	RNA_POL_BETA RNA polymerases beta chain signature. GdKFASrHGQKGV

Chain	Residue	Details
B	GLY878-VAL890

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00615, ECO:0000269\|PubMed:19419240, ECO:0007744\|PDB:2WAQ, ECO:0007744\|PDB:2WB1, ECO:0007744\|PDB:4AYB, ECO:0007744\|PDB:4V8S

Chain	Residue	Details
P	CYS9
Y	LYS221
Y	GLN328
Y	GLU348
X	CYS9
D	CYS209
S	CYS203
S	CYS206
S	CYS209
Y	GLU109
Y	LYS148
Y	LYS192

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269\|PubMed:19419240, ECO:0007744\|PDB:2WAQ, ECO:0007744\|PDB:2WB1, ECO:0007744\|PDB:2Y0S, ECO:0007744\|PDB:4AYB, ECO:0007744\|PDB:4V8S

Chain	Residue	Details
P	THR12
X	THR12
B	CYS1082
R	CYS1064
R	CYS1067
R	CYS1082

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00615, ECO:0000269\|PubMed:19419240, ECO:0007744\|PDB:2WAQ, ECO:0007744\|PDB:2WB1, ECO:0007744\|PDB:2Y0S, ECO:0007744\|PDB:4AYB, ECO:0007744\|PDB:4V8S

Chain	Residue	Details
P	CYS26
W	LYS88
W	GLU92
W	LYS138
W	LYS303
W	LYS305
W	ARG323
W	GLN422
W	ARG812
W	GLN815
P	CYS29
X	CYS26
X	CYS29
A	LYS305
A	ARG323
A	GLN422
A	ARG812
A	GLN815

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00863, ECO:0000269\|PubMed:19419240, ECO:0007744\|PDB:2WAQ, ECO:0007744\|PDB:2Y0S, ECO:0007744\|PDB:4AYB, ECO:0007744\|PDB:4V8S

Chain	Residue	Details
A	CYS98
A	CYS101
W	CYS98
W	CYS101

site_id	SWS_FT_FI5
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00863, ECO:0000269\|PubMed:19419240, ECO:0007744\|PDB:2WAQ, ECO:0007744\|PDB:2Y0S, ECO:0007744\|PDB:4AYB

Chain	Residue	Details
A	CYS146
W	CYS146

site_id	SWS_FT_FI6
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00863, ECO:0007744\|PDB:4AYB, ECO:0007744\|PDB:4V8S

Chain	Residue	Details
A	CYS149
W	CYS149

site_id	SWS_FT_FI7
Number of Residues	6
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00863, ECO:0000269\|PubMed:19419240, ECO:0007744\|PDB:2WAQ, ECO:0007744\|PDB:2WB1, ECO:0007744\|PDB:2Y0S, ECO:0007744\|PDB:4AYB, ECO:0007744\|PDB:4V8S

Chain	Residue	Details
A	ASP456
A	ASP458
A	ASP460
W	ASP456
W	ASP458
W	ASP460

site_id	SWS_FT_FI8
Number of Residues	8
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P11512

Chain	Residue	Details
A	ARG573
A	CYS575
A	CYS580
A	HIS582
W	ARG573
W	CYS575
W	CYS580
W	HIS582

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)