2XZP
Upf1 helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 146.120, 146.120, 146.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 84.362 - 2.720 |
| R-factor | 0.1751 |
| Rwork | 0.173 |
| R-free | 0.21410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wjv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.785 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.360 | 2.870 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rmerge | 0.060 | 0.640 |
| Number of reflections | 28118 | |
| <I/σ(I)> | 32.2 | 4.4 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 13.4 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 50MM SODIUM CITRATE PH 6.0, 1.5M SODIUM MALONATE |
