2XZL
Upf1-RNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-11-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.135, 114.117, 65.730 |
| Unit cell angles | 90.00, 110.24, 90.00 |
Refinement procedure
| Resolution | 41.882 - 2.400 |
| R-factor | 0.1999 |
| Rwork | 0.197 |
| R-free | 0.24310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wjv |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.667 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.200 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 0.300 |
| Number of reflections | 33993 | |
| <I/σ(I)> | 8.3 | 3.2 |
| Completeness [%] | 98.1 | 98.8 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 50MM MES PH 6.0, 200MM AMMONIUM ACETATE, 20% PEG 3350 |
