2XYS
Crystal structure of Aplysia californica AChBP in complex with strychnine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 100.593, 100.593, 289.676 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.608 - 1.909 |
| R-factor | 0.1738 |
| Rwork | 0.172 |
| R-free | 0.20970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uz6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.194 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.560 | 2.010 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.080 | 0.620 |
| Number of reflections | 113796 | |
| <I/σ(I)> | 13.9 | 2.3 |
| Completeness [%] | 98.2 | 91.7 |
| Redundancy | 5.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 MM SODIUM ACETATE, 100 MM BISTRISPROPANE AT PH 8.5, 15.5% PEG3350 |
