2XYO
Structural basis for a new tetracycline resistance mechanism relying on the TetX monooxygenase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97784, 0.97838, 0.95369 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.345, 68.658, 152.483 |
| Unit cell angles | 90.00, 101.08, 90.00 |
Refinement procedure
| Resolution | 149.640 - 3.000 |
| R-factor | 0.25132 |
| Rwork | 0.249 |
| R-free | 0.29454 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.519 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 3.190 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.220 | 0.730 |
| Number of reflections | 35742 | |
| <I/σ(I)> | 11.7 | 2.9 |
| Completeness [%] | 99.5 | 98.8 |
| Redundancy | 6.9 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 1.4 M AMMONIUM SULFATE, 100 MM SODIUM CITRATE BUFFER PH 7 AT 293 K |
