2XWA

Crystal Structure of Complement Factor D Mutant R202A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-05-15
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	44.222, 67.491, 133.588
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.800
R-factor	0.23982
Rwork	0.238
R-free	0.28297
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dsu
RMSD bond length	0.008
RMSD bond angle	1.241
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.490	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.070	0.200
Number of reflections	10248
<I/σ(I)>	14.5	4.7
Completeness [%]	98.6	94.5
Redundancy	3.4	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		pH 6.0