2XSZ
The dodecameric human RuvBL1:RuvBL2 complex with truncated domains II
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-12 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 111.824, 187.925, 244.889 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.140 - 3.000 |
| R-factor | 0.1795 |
| Rwork | 0.178 |
| R-free | 0.20540 |
| Structure solution method | MAD |
| Starting model (for MR) | 2c9o |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.080 | 0.760 |
| Number of reflections | 51774 | |
| <I/σ(I)> | 12 | 1.7 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.2 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | CRYSTALLIZATION DROPS WERE MIXED FROM EQUAL VOLUMES OF PROTEIN SOLUTION (12 MG/ML, 20MM TRIS-HCL PH 8.0, 200MM NACL, 10% GLYCEROL, 4MM MGCL2, 4MM ADP, 0.5MM TCEP) AND CRYSTALLIZATION SOLUTION (0.2M MGCL2, 30% PEG 400, 0.1M HEPES PH 7.5). |
