2XSG
Structure of the gh92 family glycosyl hydrolase ccman5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 82.202, 91.158, 224.523 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.800 - 2.000 |
| R-factor | 0.19303 |
| Rwork | 0.191 |
| R-free | 0.23861 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.237 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.800 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.160 | 0.400 |
| Number of reflections | 106820 | |
| <I/σ(I)> | 7.8 | 3.7 |
| Completeness [%] | 97.6 | 89.6 |
| Redundancy | 5.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M NAF, 0.1 M BIS-TRIS PROPANE PH 7.5, 20% PEG 3350 |
