2XS0
Linear binding motifs for JNK and for calcineurin antagonistically control the nuclear shuttling of NFAT4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-18 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.530, 106.550, 130.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.883 - 2.600 |
| R-factor | 0.1938 |
| Rwork | 0.188 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xrw |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.811 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.900 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.070 | 0.870 |
| Number of reflections | 13792 | |
| <I/σ(I)> | 16.6 | 2.06 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 24-26% PEG1000, 100MM TRIS PH 8.5 |
