2XRY
X-ray structure of archaeal class II CPD photolyase from Methanosarcina mazei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 69.770, 69.770, 243.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.500 |
| R-factor | 0.18523 |
| Rwork | 0.185 |
| R-free | 0.21017 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UNPUBLISHED STRUCTURAL DATA OF M. BARKERI PHOTOLYASE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.378 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.940 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.390 |
| Number of reflections | 95566 | |
| <I/σ(I)> | 11.8 | 4.4 |
| Completeness [%] | 98.4 | 98.8 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.5 M LITHIUM SULFATE, 7.5% (W/V) PEG 8000 |
