2XNM
Structure of NEK2 bound to CCT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-23 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.800, 56.830, 81.300 |
Unit cell angles | 90.00, 133.21, 90.00 |
Refinement procedure
Resolution | 36.734 - 1.850 |
R-factor | 0.1649 |
Rwork | 0.163 |
R-free | 0.19620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.022 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.170 | 1.890 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmerge | 0.060 | 0.400 |
Number of reflections | 27237 | |
<I/σ(I)> | 11.1 | 2.5 |
Completeness [%] | 98.1 | 99.1 |
Redundancy | 2.6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |