2XNJ
Crystal structure of an engineered Ferredoxin(flavodoxin) NADP(H) Reductase (FPR) from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 108 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-12 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.883, 74.580, 44.421 |
| Unit cell angles | 90.00, 101.86, 90.00 |
Refinement procedure
| Resolution | 27.820 - 1.900 |
| R-factor | 0.1889 |
| Rwork | 0.188 |
| R-free | 0.22360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fdr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.420 |
| Number of reflections | 45215 | |
| <I/σ(I)> | 12.8 | 3.2 |
| Completeness [%] | 95.8 | 92.1 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
