2XL2
WDR5 IN COMPLEX WITH AN RBBP5 PEPTIDE RECRUITED TO NOVEL SITE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2009-09-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.440, 81.260, 87.520 |
| Unit cell angles | 90.00, 93.12, 90.00 |
Refinement procedure
| Resolution | 43.695 - 2.400 |
| R-factor | 0.1884 |
| Rwork | 0.185 |
| R-free | 0.24250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h13 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.128 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.500 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 0.530 |
| Number of reflections | 23706 | |
| <I/σ(I)> | 9.4 | 2.3 |
| Completeness [%] | 98.4 | 98 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M TRIS HCL PH 8.0, 25% PEG 8000 |
