2XH2
Engineering the enolase active site pocket: Crystal structure of the S39N D321A mutant of yeast enolase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-10-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.720, 82.540, 95.610 |
| Unit cell angles | 89.41, 71.31, 84.80 |
Refinement procedure
| Resolution | 35.650 - 1.800 |
| R-factor | 0.16797 |
| Rwork | 0.166 |
| R-free | 0.20949 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xh0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.181 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.900 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.130 | 0.490 |
| Number of reflections | 162601 | |
| <I/σ(I)> | 12.74 | 3.48 |
| Completeness [%] | 93.1 | 87.3 |
| Redundancy | 2.01 | 1.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 18 % PEG 3350, 0.2 M POTASSIUM SULFATE |
