2XE0
Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS |
| Synchrotron site | SLS |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 177.857, 71.538, 45.005 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.769 - 2.310 |
| R-factor | 0.2152 |
| Rwork | 0.213 |
| R-free | 0.25570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9z |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.309 |
| Data reduction software | XDS |
| Data scaling software | XSCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.760 | 35.760 |
| High resolution limit [Å] | 2.310 | 2.400 |
| Rmerge | 0.440 | 0.060 |
| Number of reflections | 24763 | |
| <I/σ(I)> | 1.1 | 12.1 |
| Completeness [%] | 74.4 | 94.9 |
| Redundancy | 2.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
