2XDV
Crystal Structure of the Catalytic Domain of FLJ14393
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-08-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 185.180, 185.180, 185.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.280 - 2.570 |
| R-factor | 0.1981 |
| Rwork | 0.197 |
| R-free | 0.22850 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | BUSTER-TNT (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.090 | 1.000 |
| Number of reflections | 35135 | |
| <I/σ(I)> | 17 | 2 |
| Completeness [%] | 99.5 | 97 |
| Redundancy | 9.4 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12% PEG 3350; 0.005M COCL2; 0.005M MGCL2; 0.005M CDCL2; 0.005M NICL2; 0.1M HEPES PH 7.5 |
