2XAC
Structural Insights into the Binding of VEGF-B by VEGFR-1D2: Recognition and Specificity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.212, 65.526, 82.824 |
| Unit cell angles | 90.00, 90.26, 90.00 |
Refinement procedure
| Resolution | 32.760 - 2.710 |
| R-factor | 0.286 |
| Rwork | 0.282 |
| R-free | 0.36400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2C7W AND 1FLT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.113 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (FOR MR) |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.850 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Rmerge | 0.120 | 0.270 |
| Number of reflections | 11510 | |
| <I/σ(I)> | 12.5 | 2.6 |
| Completeness [%] | 85.0 | 47.8 |
| Redundancy | 11.8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 14% PEG 4000, 0.1M SODIUM CITRATE, PH 5.6 AND 0.2M LICL |
