2XA0
Crystal structure of BCL-2 in complex with a BAX BH3 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 93 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 81.973, 81.973, 138.850 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.700 |
R-factor | 0.2223 |
Rwork | 0.222 |
R-free | 0.24220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g5m |
RMSD bond length | 0.009 |
RMSD bond angle | 1.363 |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.080 | 0.220 |
Number of reflections | 14540 | |
<I/σ(I)> | 30.4 | 4.5 |
Completeness [%] | 93.9 | 75.3 |
Redundancy | 9.7 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M SODIUM ACETATE (PH 4.2), 3.0 M SODIUM FORMATE |