2X8J
Intracellular subtilisin precursor from B. clausii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 125.659, 125.659, 106.141 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.980 - 1.560 |
| R-factor | 0.18961 |
| Rwork | 0.187 |
| R-free | 0.22973 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wwt |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.215 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 108.400 | 1.640 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.077 | 1.080 |
| Number of reflections | 269002 | |
| <I/σ(I)> | 13.4 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | PEG 4K GLYCEROL 0.1M SODIUM CACODYLATE PH 5.9 0.2M SODIUM ACETATE. |
