2X6N
Human foamy virus integrase - catalytic core. Manganese-bound structure.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2009-01-28 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.767, 89.232, 177.084 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.620 - 2.060 |
| R-factor | 0.22793 |
| Rwork | 0.226 |
| R-free | 0.27004 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x74 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.085 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.230 | 2.170 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.070 | 0.700 |
| Number of reflections | 78830 | |
| <I/σ(I)> | 14.2 | 2 |
| Completeness [%] | 93.8 | 71 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.8-2M AMMONIUM FORMATE 100MM HEPES, PH 7.5 5MM MNCL2 10-15% GLYCEROL |
