2X5J
Crystal structure of the Apoform of the D-Erythrose-4-phosphate dehydrogenase from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.300, 110.700, 138.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.500 - 2.300 |
| R-factor | 0.20921 |
| Rwork | 0.206 |
| R-free | 0.26636 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gd1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.468 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.500 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.430 |
| Number of reflections | 59159 | |
| <I/σ(I)> | 24.4 | 5.6 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10 % (W/V) PEG 8000, 100 MM NA/K PHOSPHATE BUFFER PH 6.2, 200 MM NACL |
