2X4T
Crystal structure of MHC CLass I HLA-A2.1 bound to a Peiodate- cleavable peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-12-08 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.391, 85.516, 80.394 |
| Unit cell angles | 90.00, 90.33, 90.00 |
Refinement procedure
| Resolution | 19.830 - 2.300 |
| R-factor | 0.1858 |
| Rwork | 0.184 |
| R-free | 0.23570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eey |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.334 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.500 | 2.420 |
| High resolution limit [Å] | 2.200 | 2.300 |
| Rmerge | 0.060 | 0.510 |
| Number of reflections | 37003 | |
| <I/σ(I)> | 7.9 | 1.4 |
| Completeness [%] | 98.1 | 97.9 |
| Redundancy | 3.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES PH 6.5, 22% PEG1500 |
