2X4M
Yersinia Pestis Plasminogen Activator Pla
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.913, 127.824, 107.175 |
| Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
| Resolution | 19.930 - 2.550 |
| R-factor | 0.186 |
| Rwork | 0.185 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.267 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.580 |
| High resolution limit [Å] | 2.550 | 2.540 |
| Rmerge | 0.100 | |
| Number of reflections | 56736 | |
| <I/σ(I)> | 1.93 | |
| Completeness [%] | 95.7 | 79.2 |
| Redundancy | 6.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.5 | 16% PEG 400, 0.1M LITHIUM CITRATE PH: 3.5 |
