2X18
The crystal structure of the PH domain of human AKT3 protein kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.475, 62.401, 71.411 |
| Unit cell angles | 111.47, 102.75, 94.36 |
Refinement procedure
| Resolution | 64.030 - 1.460 |
| R-factor | 0.19156 |
| Rwork | 0.189 |
| R-free | 0.23377 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1unp |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.658 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.030 | 1.540 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.080 | 0.470 |
| Number of reflections | 164858 | |
| <I/σ(I)> | 10 | 2 |
| Completeness [%] | 98.1 | 94.3 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M PROLINE, 0.1M HEPES PH 7.5, 10% PEG3350 |
