2X11

Crystal structure of the complete EphA2 ectodomain in complex with ephrin A5 receptor binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-13
Detector	ADSC QUANTUM 4
Spacegroup name	P 2 2 21
Unit cell lengths	173.630, 59.630, 112.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.482 - 4.830
R-factor	0.313
Rwork	0.312
R-free	0.31430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3CZU 3fl7 1shw
RMSD bond length	0.003
RMSD bond angle	0.675
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	173.000	4.900
High resolution limit [Å]	4.800	4.800
Rmerge	0.110	0.740
Number of reflections	6016
<I/σ(I)>	12.95	2.8
Completeness [%]	98.2	75.7
Redundancy	6.2	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				TWO PARTS OF PROTEIN SOLUTION WERE MIXED WITH ONE PART WATER, ONE PART RESERVOIR SOLUTION (8 % POLYETHYLENE GLYCOL 6000, 0.8 M LICL, 0.08 M CITRATE PH 5) AND ONE PART 1 % POLYVINYLPYRROLIDONE K15.