2X0N

Structure of glycosomal glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma brucei determined from Laue data

Replaces:  1GGA

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.7
Synchrotron site	SRS
Beamline	PX9.7
Temperature [K]	273
Detector technology	FILM
Detector	CEA
Wavelength(s)	0.489-1.905
Spacegroup name	P 21 21 2
Unit cell lengths	135.520, 256.270, 114.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.399 - 3.200
R-factor	0.1533
Rwork	0.150
R-free	0.21820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gd1
RMSD bond length	0.009
RMSD bond angle	1.492
Data reduction software	DARESBURY (LAUE PROCESSING SOFTWARE)
Data scaling software	DARESBURY (LAUE PROCESSING SOFTWARE)
Phasing software	MERLOT
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	7.400
High resolution limit [Å]	3.200
Rmerge	0.150
Number of reflections	34991
<I/σ(I)>	7.2
Completeness [%]	57.0
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		PROTEIN: 5 MICROL OF 6 MG/ML GGAPDH IN 25 MM TRIS HCL PH 7.8, 0.6M AMMONIUM SULPHATE, 2MM NAD, 1MM DITHIOTHREITOL 1MM NA AZIDE, 1 MM EDTA. PRECIPITANT: 5 MICROL OF 52% SATURATED AMMONIUM SULPHATE, 0.2M N,N-BIS-(2-HYDROXYETHYL)-2-AMINOETHANE SULFONIC ACID PH 6.5, 1MM DITHIOTHREITOL, 1MM NA AZIDE, 1MM EDTA, 20MM 3-PHOSPHOGLYCERATE. RESERVOIR: 1 ML