2X0B
Crystal structure of human angiotensinogen complexed with renin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-31 |
Detector | ADSC CCD |
Spacegroup name | H 3 |
Unit cell lengths | 212.475, 212.475, 474.061 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.520 - 4.330 |
R-factor | 0.31 |
Rwork | 0.309 |
R-free | 0.33400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wxw |
RMSD bond length | 0.006 |
RMSD bond angle | 1.158 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: DEV_249)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.500 | 4.590 |
High resolution limit [Å] | 4.350 | 4.350 |
Rmerge | 0.130 | 0.860 |
Number of reflections | 6793 | |
<I/σ(I)> | 3.6 | 1 |
Completeness [%] | 97.2 | 89.2 |
Redundancy | 2.4 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 1.6-2.2M AS, 0.1M MES, PH6 |