2WXH
The crystal structure of the murine class IA PI 3-kinase p110delta in complex with SW14.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-05 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.870, 65.010, 117.080 |
| Unit cell angles | 90.00, 103.58, 90.00 |
Refinement procedure
| Resolution | 38.040 - 1.900 |
| R-factor | 0.21824 |
| Rwork | 0.217 |
| R-free | 0.24504 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rd0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.262 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0053) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.110 | 1.920 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.640 |
| Number of reflections | 81877 | |
| <I/σ(I)> | 12.66 | 1.63 |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 3.59 | 3.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 20% (V/V) GLYCEROL, 10% (W/V) PEG 4K, 30 MM NANO3, 30 MM NA2HPO4, 30 MM (NH4)2SO4, 100 MM IMIDAZOLE PH 6.8 |
