2WWW

Crystal Structure of Methylmalonic Acidemia Type A Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-15
Detector	ADSC CCD
Spacegroup name	P 42
Unit cell lengths	149.430, 149.430, 69.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.430 - 2.640
R-factor	0.184
Rwork	0.182
R-free	0.22190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.350
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.430	2.780
High resolution limit [Å]	2.640	2.640
Rmerge	0.070	0.610
Number of reflections	45149
<I/σ(I)>	10.2	2
Completeness [%]	99.8	100
Redundancy	4	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				CUSTOM.