2WWR
Crystal Structure of Human Glyoxylate Reductase Hydroxypyruvate Reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-14 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.356, 116.080, 198.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.000 - 2.820 |
R-factor | 0.22036 |
Rwork | 0.217 |
R-free | 0.27857 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gcg |
RMSD bond length | 0.007 |
RMSD bond angle | 1.073 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.180 | 0.790 |
Number of reflections | 34609 | |
<I/σ(I)> | 10.5 | 2 |
Completeness [%] | 91.0 | 87.6 |
Redundancy | 11.1 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1M SODIUM CACODYLATE PH 6.75, 10% PEG 8K, 0.25M MAGNESIUM ACETATE TETRAHYDRATE |