2WWP
Crystal structure of the human lipocalin-type prostaglandin D synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.400, 56.590, 73.460 |
| Unit cell angles | 90.00, 90.15, 90.00 |
Refinement procedure
| Resolution | 32.650 - 2.000 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2czt |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.104 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.650 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.125 | 0.460 |
| Number of reflections | 20007 | |
| <I/σ(I)> | 7.8 | 2.8 |
| Completeness [%] | 98.3 | 89.8 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20% PEG3350, 0.2 M SODIUM THIOCYANATE, pH 7 |
