2WVT
Crystal structure of an alpha-L-fucosidase GH29 from Bacteroides thetaiotaomicron in complex with a novel iminosugar fucosidase inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2009-04-18 |
| Detector | ADSC CCD |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 104.830, 85.160, 121.285 |
| Unit cell angles | 90.00, 108.73, 90.00 |
Refinement procedure
| Resolution | 90.930 - 1.800 |
| R-factor | 0.20936 |
| Rwork | 0.207 |
| R-free | 0.24508 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wvv |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.349 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.100 |
| Number of reflections | 93456 |
| <I/σ(I)> | 8.7 |
| Completeness [%] | 99.9 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
