2WVQ
Structure of the HET-s N-terminal domain. Mutant D23A, P33H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.330, 83.150, 67.360 |
| Unit cell angles | 90.00, 104.55, 90.00 |
Refinement procedure
| Resolution | 65.230 - 2.000 |
| R-factor | 0.22725 |
| Rwork | 0.225 |
| R-free | 0.26947 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wvn |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.739 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.460 |
| Number of reflections | 34078 | |
| <I/σ(I)> | 10.5 | 3.7 |
| Completeness [%] | 95.2 | 93.3 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 276 | CRYSTALLIZED AT 276K IN 25% ETHYLENE GLYCOL |
