2WVF
Structural and mechanistic insights into Helicobacter pylori NikR function
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-06 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 70.534, 70.534, 226.188 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.081 - 1.600 |
| R-factor | 0.1854 |
| Rwork | 0.185 |
| R-free | 0.19950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cad |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.998 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.080 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.510 |
| Number of reflections | 44866 | |
| <I/σ(I)> | 8.5 | 1.5 |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 7.6 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.4 TO 0.7 M AMMONIUM SULFATE AND 100 MM CITRATE PH 5-5.4 |
