2WUB
Crystal structure of HGFA in complex with the allosteric non- inhibitory antibody Fab40.deltaTrp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.356, 89.530, 118.470 |
| Unit cell angles | 90.00, 91.08, 90.00 |
Refinement procedure
| Resolution | 19.990 - 2.900 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r0l |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.090 | 0.500 |
| Number of reflections | 33747 | |
| <I/σ(I)> | 14 | 2.7 |
| Completeness [%] | 98.8 | 98.3 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 10% PEG 10,000, 100 MM HEPES PH 7.5 |
