2WU7
Crystal Structure of the Human CLK3 in complex with V25
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-03-16 |
Detector | BRUKER |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.870, 62.200, 75.370 |
Unit cell angles | 90.00, 97.97, 90.00 |
Refinement procedure
Resolution | 28.940 - 2.250 |
R-factor | 0.1934 |
Rwork | 0.190 |
R-free | 0.24560 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.070 |
Data reduction software | SADABS |
Data scaling software | SAINT |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.320 | 2.290 |
High resolution limit [Å] | 2.240 | 2.240 |
Rmerge | 0.060 | 0.580 |
Number of reflections | 19231 | |
<I/σ(I)> | 7.26 | 2.12 |
Completeness [%] | 99.8 | 96.2 |
Redundancy | 6.59 | 4.42 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2M (NH4)2SO4, 0.1M BIS-TRIS PH 5.5, 25% PEG 3350 |