2WU6
Crystal Structure of the Human CLK3 in complex with DKI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-04-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.150, 62.330, 74.150 |
| Unit cell angles | 90.00, 96.04, 90.00 |
Refinement procedure
| Resolution | 73.740 - 1.920 |
| R-factor | 0.17215 |
| Rwork | 0.170 |
| R-free | 0.21137 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.449 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.320 | 2.020 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.050 | 0.760 |
| Number of reflections | 31032 | |
| <I/σ(I)> | 7.3 | 2 |
| Completeness [%] | 97.1 | 96.2 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M (NH4)2SO4, 0.1M BIS-TRIS PH 5.5, 25% PEG 3350 |
