2WTT

Structure of the human p73 tetramerization domain (crystal form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	56.120, 84.000, 169.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.786 - 2.300
R-factor	0.2333
Rwork	0.230
R-free	0.28740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wqi
RMSD bond length	0.009
RMSD bond angle	1.176
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.700	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.090	0.354
Number of reflections	36567
<I/σ(I)>	15.7	5.9
Completeness [%]	100.0	100
Redundancy	11.6	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	290	SITTING DROP VAPOR DIFFUSION AT 17 DEGREE C. PROTEIN SOLUTION: 15 MG/ML IN 20 MM TRIS (PH 8.5), 50 MM NACL. CRYSTALLIZATION BUFFER: 0.1 M SODIUM CITRATE (PH 6.2), 40% PEG 600.