2WT7

Crystal structure of the bZIP heterodimeric complex MafB:cFos bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-06-05
Detector	MAR555 FLAT PANEL
Spacegroup name	P 62
Unit cell lengths	146.676, 146.676, 51.117
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	127.000 - 2.300
R-factor	0.22967
Rwork	0.228
R-free	0.25579
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.009
RMSD bond angle	1.391
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXD
Refinement software	REFMAC (5.4.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.400	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.050	0.320
Number of reflections	28122
<I/σ(I)>	10.1	2.4
Completeness [%]	99.5	99.3
Redundancy	11.9	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1
1