2WSQ
MonoTIM mutant RMM0-1, dimeric form.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-16 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.882, 94.082, 108.885 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.250 - 2.100 |
R-factor | 0.20813 |
Rwork | 0.204 |
R-free | 0.24377 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tri |
RMSD bond length | 0.020 |
RMSD bond angle | 1.586 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.140 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.060 | 0.360 |
Number of reflections | 53956 | |
<I/σ(I)> | 9.1 | 2 |
Completeness [%] | 98.2 | 98.5 |
Redundancy | 4.3 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.2 | 303 | 100 MM MES BUFFER PH 6.2, 180 MM LI2SO4, 26 PERCENT PEG 6000, 5 MM DITHIOTHREITOL, 1 MM EDTA AND 1 MM NAN3, AT 30 DEGREES CELSIUS. |