2WQ9
Crystal Structure of RBP4 bound to Oleic Acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 105 |
| Spacegroup name | H 3 |
| Unit cell lengths | 103.060, 103.060, 72.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.340 - 1.650 |
| R-factor | 0.18924 |
| Rwork | 0.188 |
| R-free | 0.21919 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qab |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.116 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.040 | 0.420 |
| Number of reflections | 34250 | |
| <I/σ(I)> | 16.96 | 2.82 |
| Completeness [%] | 98.8 | 99.8 |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.5 TO 4.2 M NACL, 1M HEPES PH 7.2 |
