2WQ8
Glycan labelling using engineered variants of galactose oxidase obtained by directed evolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 88.464, 88.464, 410.846 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 76.700 - 2.190 |
| R-factor | 0.18515 |
| Rwork | 0.182 |
| R-free | 0.24175 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1GOF AND 2EID |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.113 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 2.230 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.070 | 0.360 |
| Number of reflections | 43048 | |
| <I/σ(I)> | 25.34 | 2.43 |
| Completeness [%] | 91.9 | 13.4 |
| Redundancy | 15.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 10% PEG6K, 0.1 M CACL2, 5% GLYCEROL, 10 MM GLCNAC, 50 M ACETATE PH 5. |
