2WPT
The crystal structure of Im2 in complex with colicin E9 DNase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-06 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.229, 82.897, 89.528 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.590 - 1.780 |
| R-factor | 0.19434 |
| Rwork | 0.193 |
| R-free | 0.22288 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fsj |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.726 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.140 | 0.440 |
| Number of reflections | 26874 | |
| <I/σ(I)> | 14.1 | 2.46 |
| Completeness [%] | 97.6 | 78.6 |
| Redundancy | 6.7 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | pH 6.8 |
