2WPK
factor IXa superactive triple mutant, ethylene glycol-soaked
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-02-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.070, 66.530, 96.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.620 - 2.210 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wph |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.460 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.600 | 2.300 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.070 | 0.260 |
| Number of reflections | 14747 | |
| <I/σ(I)> | 22.9 | 6.7 |
| Completeness [%] | 99.7 | 86.6 |
| Redundancy | 6.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 22 % PEG 3000 100 MM BIS/TRIS PH 6.85 |
