2WOJ
ADP-AlF4 complex of S. cerevisiae GET3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.612, 82.628, 167.282 |
| Unit cell angles | 90.00, 98.28, 90.00 |
Refinement procedure
| Resolution | 37.441 - 1.994 |
| R-factor | 0.1778 |
| Rwork | 0.176 |
| R-free | 0.21270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PARTIAL MODEL DERIVED FROM SAD DATASET |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.331 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.590 |
| Number of reflections | 94446 | |
| <I/σ(I)> | 21.1 | 1.9 |
| Completeness [%] | 95.9 | 86.3 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.4 | 33% PEG3350, 0.1 M TRIS PH 8.4, 0.2 M AMMONIUM ACETATE, 75 MM NA/K TARTRATE |
