2WO8
MMP12 complex with a beta hydroxy carboxylic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.043, 65.264, 67.808 |
| Unit cell angles | 66.47, 83.86, 71.37 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.18728 |
| Rwork | 0.184 |
| R-free | 0.24589 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.567 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.4.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.310 |
| Number of reflections | 45242 | |
| <I/σ(I)> | 10.4 | 2.35 |
| Completeness [%] | 97.1 | 90.5 |
| Redundancy | 1.94 | 1.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 100MM MES PH 5.2-5.6, 16-20% PEG 5KMME, 200MM AMMONIUM SULPHATE |
