2WO3
Crystal Structure of the EphA4-ephrinA2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 64 |
| Unit cell lengths | 115.412, 115.412, 59.354 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.970 - 2.350 |
| R-factor | 0.20012 |
| Rwork | 0.198 |
| R-free | 0.24554 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kgy |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.287 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.090 | 0.750 |
| Number of reflections | 18961 | |
| <I/σ(I)> | 30 | 1.8 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 16 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 20% PEG 3350, 200 MM KNO3, pH 8.0 |
