2WNP
M-ficolin mutant Y271F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 77.36 |
| Collection date | 2008-11-29 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 73.550, 73.550, 124.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.540 - 1.210 |
| R-factor | 0.17839 |
| Rwork | 0.178 |
| R-free | 0.19137 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jhm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.108 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.240 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.050 | 0.580 |
| Number of reflections | 54807 | |
| <I/σ(I)> | 28.27 | 2.37 |
| Completeness [%] | 72.0 | 7.3 |
| Redundancy | 9.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
