2WNH
Crystal Structure Analysis of Klebsiella sp ASR1 Phytase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 133.680, 133.680, 111.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.490 - 1.680 |
| R-factor | 0.18249 |
| Rwork | 0.181 |
| R-free | 0.20744 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.315 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (RESOLVE) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.490 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.070 | 0.410 |
| Number of reflections | 113622 | |
| <I/σ(I)> | 15 | 2.6 |
| Completeness [%] | 94.1 | 72.9 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 M SODIUM FORMATE |
